Microscope Use

1 Ocular knob
2 Body Tube
3 Arm
4 Mechanical stage knob
5 Coarse adjustment knob
6 Fine adjustment knob
7 Base
8 Substage lamp
9 Diaphragm
10 Condenser
11 Stage clip of mechanical stage
12 Stage
13 Objectives
14 Revolving nosepiece
15 Nosepiece
Quick and Easy Steps for Use of the Microscope
- Carry the scope with both hands, one on the arm, one on the base.
- Using the Coarse Adjustment Knob (CA knob) , move the stage all the way down. (This prevents damage to the objectives.)
- Using lens paper ONLY, clean all pieces of glass: the oculars, objectives, condenser lens, and light source.
- Using the condenser knob, move the condenser lens upward until it is almost flush ( at the same level) with the stage.
- NOW plug in your scope and turn on the light switch.
- Using the diaphragm lever or diaphragm dial (located under the stage), open the diaphragm.
- Using the rotating nosepiece, move the 4X objective into place.
- Place the microscope slide FLAT on the stage.
- Holding the mechanical stage clip open, SLIDE the microscope slide into the mechanical stage holder.
- Looking directly at the stage (DO NOT look through the oculars!), center the specimen or cover slip directly over the brightly-lit condenser lens. Use your mechanical stage knobs to do this.
- Using the CA knob, move the stage all the way up until it comes to the Automatic Stop. (Only the 4X lens has this Auto Stop.)
- NOW (But not before now), look in the oculars.
- SLOWLY move the CA knob downward until you come into focus. (Congratulations!)
- Once you are in focus on 4X, YOU ARE AUTOMATICALLY IN FOCUS ON ALL OTHER POWERS! (This is called Parfocalization.)
- DO NOT touch the CA knob anymore, or you will lose your focus. Simply dial-in any objective you wish.
- If you need to re-focus, use ONLY the Fine Adjustment Knob.
- At higher powers, your specimen may be larger than your field of view. You may have to "hunt" for it using your mechanical stage knobs.
- To use the oil immersion power, you MUST put ONE drop of oil on the slide. Dial "in-between" any two objectives to do this.
CLEAN-UP
- BEFORE you remove any slide, always return to 4X power.
- Clean any oil off the slide and return it to its proper tray.
- Clean any oil off the objectives. Use only lens paper.
- Turn off the light switch; unplug the cord; WRAP the cord around the scope.
- Store the microscope on 4X power ONLY.